Plasmid

Part:BBa_K2061002:Design

Designed by: avi matityahu   Group: iGEM16_Tel-Hai   (2016-08-23)


gRNA1 for editing F508 deletion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 431
    Illegal PstI site found at 466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part cloned between EcoRI and PstI sites.



Source

synthetic production of the part


References